Bryan Thines
Assistant Professor of Biology
 
Email: bthines@kecksci.claremont.edu
Office: Keck Science Center 212
Phone: 909-607-8196
Office Hours: Tues 9:00 - 11:00 am, Thurs 10:30 - 12:00 pm
Web Site:
   
Educational Background:
Post-doc Plant Gene Expression Center, UC Berkeley
Ph.D. Washington State University, Molecular Plant Sciences
B.S. SUNY at Plattsburgh, Biochemistry
Courses Taught:
BIOL040L Introduction to Biological Chemistry
BIOL043L Introductory Biology
BIOL156L Genomics and Bioinformatics
Research Interests:
The ubiquitin / 26S proteasome system (UPS) is a major pathway accomplishing cellular change in eukaryotes. The UPS is required for important biological processes ranging from hormone signaling in plants to cell cycle progression in humans, where its mis-regulation has been associated with some types of cancer. The UPS alters the cellular state by selective removal of target proteins, which can be key repressors or activators of gene expression. Within the UPS, a ubiquitin ligase marks targets for removal by attaching ubiquitin, a small 76-residue protein. The SCF complex is one type of ubiquitin ligase composed of three core subunits plus an F-box protein. F-box proteins are interchangeable, and they specifically interact with and recruit diverse targets to the core complex for ubiquitylation and consequent removal by the 26S proteasome. Remarkably, plant genomes are highly enriched in F-box genes relative to other eukaryotes, with approximately 700 encoded in the model plant Arabidopsis thaliana. This enrichment implies both a broad target range and a heavy reliance on the UPS in plant-specific processes and, furthermore, establishes plants as a particularly good system in which to study the UPS. The overwhelming majority of these predicted F-box proteins are of unknown biological function. Because plants require distinctive strategies to survive environmental stresses, our global hypothesis is that many of these F-box proteins help alter the physiological state of the plant cell and assist in stress adaptation. The immediate goal of my lab is to identify specific F-box genes acting under defined stresses that could help plants survive adverse environmental conditions.

We use both functional genomic and molecular genetic approaches to identify and then characterize F-box genes that potentially act in stress responses. Our findings contribute to the understanding of how plants deal with environmental stress, as well as to how the UPS works in eukaryotic cells.
Thesis Topics:
 
Selected Publications List: Click to open new window.
1.   Thines B, Youn, Duarte M, and Harmon FG . (2014). The time of day effects of warm temperature on flowering time involve PIF4 and PIF5. Journal of Experimental Botany   : .
 
2.   Thines B, Mandaokar A, and Browse J . (2013). Characterizing jasmonate regulation of male fertility in Arabidopsis. Methods Mol Biol.   1011: 13-23.
 
3.   Thines B, Harmon FG . (2011). Four easy pieces: mechanisms underlying circadian regulation of growth and development. Curr Opin Plant Biol   14 (1): 31-7.
 
4.   Thines B, Harmon FG . (2010). Ambient temperature response establishes ELF3 as a required component of the core Arabidopsis circadian clock. Proc Natl Acad Sci   107 (7): 3257-62.
 
5.   Melotto M, Mecey C, Niu Y, Chung HS, Katsir L, Yao J, Zeng W, Thines B, Staswick P, Browse J, Howe GA, He SY . (2008). A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine- and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein. Plant J   55 (6): 979-88.
 
6.   Chung HS, Koo AJ, Gao X, Jayanty S, Thines B, Jones AD, Howe GA . (2008). Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in response to wounding and herbivory. Plant Physiol   146 (3): 952-64.
 
7.   Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J . (2007). JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling. Nature   448 (7154): 661-5.
 
8.   Mandaokar A, Thines B, Shin B, Lange BM, Choi G, Koo YJ, Yoo YJ, Choi YD, Choi G, Browse J . (2006). Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling. Plant J   46 (6): 984-1008.